Protocols for working with Nymphaea thermarum
1) How to Grow Nymphaea thermarum -
- Friedman Lab Protocol (pdf, updated 2019)
- Details on how plants are grown at Royal Botanic Gardens at Kew (pdf)
2) Controlled pollinations (including emasculation) - coming soon!
3) DNA extraction -
4) RNA extractions -
5) Imaging ovules and seeds with confocal microscopy: sample preparation and imaging
- Poster with overview of sample preparation and imaging process (pdf)
- Check-list style protocol of sample preparation with Feulgen Staining (pdf)